Separation of Bovine Serum Albumin by Foam Fractionation with Sieve Tray Columnby Zhiqiang Li, Huijie Zheng, Zhaoliang Wu

Separation Science and Technology

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Download by: [University of Nebraska, Lincoln] Date: 21 September 2015, At: 02:35

Separation Science and Technology

ISSN: 0149-6395 (Print) 1520-5754 (Online) Journal homepage: http://www.tandfonline.com/loi/lsst20

Separation of Bovine Serum Albumin by Foam

Fractionation with Sieve Tray Column

Zhiqiang Li, Huijie Zheng & Zhaoliang Wu

To cite this article: Zhiqiang Li, Huijie Zheng & Zhaoliang Wu (2015): Separation of Bovine

Serum Albumin by Foam Fractionation with Sieve Tray Column, Separation Science and

Technology, DOI: 10.1080/01496395.2015.1066809

To link to this article: http://dx.doi.org/10.1080/01496395.2015.1066809

Accepted online: 16 Jul 2015.

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Ac ce pte d M an us cri pt 1

Separation of Bovine Serum Albumin by Foam

Fractionation with Sieve Tray Column

Zhiqiang Li, Huijie Zheng, Zhaoliang Wu* (School of Chemical Engineering, Hebei University of Technology, Tianjin 300130, China)

Abstract: A batch foam fractionation column with sieve trays was developed for enhancing the foam drainage. Effects of feeding concentration, air flow rate, number of stages, and sieve pore size on foam fractionation performances were investigated. The results indicated that the sieve trays enhanced the foam drainage effectively and improved enrichment ratio. At the liquid loading volume 490 mL, feeding concentration 0.1 g L−1 and air flow rate 300 mL min−1, enrichment ratio reached 7.56 by using 10 stages of sieve trays with opening ratio of 38.25%, which was 1.84 times of that obtained by the control column.

Key words: foam fractionation; sieve tray; Bovine serum albumin (BSA); enrichment ratio; recovery percentage

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Ac ce pte d M an us cri pt 2

INTRODUCTION

With more people realizing the importance of environment protection and food safety, it is interesting for green and low-cost separation techniques to be developed for the application of industries. Foam fractionation is one of these techniques with the advantages of low energy consumption, pollution free and high efficiency (1). It has been widely studied in the removal of hazardous materials such as heavy metal ions (2, 3) and dyes (4, 5). Many researchers have engaged in the study of foam fractionation in biochemical engineering for the separation of biomolecules such as proteins (6), enzymes (7) and other natural products (8).

Foam fractionation is a physical process in which the rising bubbles serve as the media for enriching or removing a surfactant from its aqueous solution. The separation performances closely depend on the two essential steps, namely interfacial adsorption and foam drainage. The former can be improved by prolonging the residence time of bubbles in the bulk liquid (9), elevating temperature (10) or designing suitable gas distributors (11) and so on. However, it is difficult to improve the foam drainage (12, 13). Thus, more researchers prefer to pay more attention to foam drainage in foam fractionation.

It has been proved that foam drainage can be enhanced by a sudden change of the area of foam flow. Deshpande et al. (14) studied the foam flow phenomena in sudden expansions and contractions. They pointed out that a substantial fraction of the liquid in the foam drains back upstream in a vertical up flow at a sudden expansion or contraction, leading to a greatly reduced liquid holdup downstream. But at a high gas flow rate, a sudden expansion eventually led to complete breakdown of the foam structure with the onset of a flooding regime. Li. et al. (15)

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Ac ce pte d M an us cri pt 3 inserted a foam riser plate in the foam phase to change the area of foam flow. The liquid holdup in the foam was significantly reduced by the riser plate and the enrichment ratio was greatly increased. However, some of the interstitial liquid between the bubbles was released from the expanding foam. In order to avoid the flooding, a hole was opened in the column wall and the drained liquid was collected and was moved out of the column immediately. Tsubomizu et al. (16) inserted perforated plates into a column for the separation of poly(vinyl alcohol) by foam fractionation with external reflux. The enrichment of poly(vinyl alcohol) could be more than double at a low gas flow rate (300 mL min−1) compared with the normal column (a column without perforated plates) and the liquid holdup in the foam was decreased. It was attributed to the enhancement of foam drainage based on the suddenly changing flow area at the perforated plates.

However, at gas flow rates above 600 mL min−1, the enrichment ratio obtained with the perforated plates decreased. It was also indicated that their results did not satisfy the material balance (15).

The reason was that the reflux foamate could not flow down from the perforated plates because of the increase of the foam flow rate induced by the sudden change of foam flow area.

In the current work, the sieves tray, which has shown excellent performances in a gas liquid contact process and has been applied in chemical industries for many years, was adopted to enhance foam drainage in a batch foam fractionation column. For there was a downcomer fixed at the sieve plate which allowed the drained liquid to fall stage by stage and finally returned to the bulk solution, the “flooding” phenomenon which has been observed by the former researchers was avoided. The principle of the sieve trays on enhancing foam drainage was presented on the basis of foam fluid mechanics. By using BSA solution as the model system, the effects of the feeding

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