QTL mapping of adult-plant resistance to stripe rust in wheat line P9897by X. L. Zhou, D. J. Han, X. M. Chen, J. M. Mu, W. B. Xue, Q. D. Zeng, Q. L. Wang, L. L. Huang, Z. S. Kang

Euphytica

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Year
2015
DOI
10.1007/s10681-015-1447-7
Subject
Agronomy and Crop Science / Plant Science / Genetics / Horticulture

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Text

QTL mapping of adult-plant resistance to stripe rust in wheat line P9897

X. L. Zhou . D. J. Han . X. M. Chen . J. M. Mu .

W. B. Xue . Q. D. Zeng . Q. L. Wang . L. L. Huang .

Z. S. Kang

Received: 6 January 2015 / Accepted: 10 April 2015  Springer Science+Business Media Dordrecht 2015

Abstract Stripe rust (or yellow rust), caused by

Puccinia striiformis f. sp. tritici (Pst), is a devastating fungal disease of common wheat. Wheat line P9897 showed adult-plant resistance (APR) to stripe rust for several years. To map resistance quantitative trait loci (QTL), F2:3 lines from a cross of P9897 with susceptible Mingxiang 169 were phenotyped for stripe rust response in replicated field trials in 2013.

Resistance gene analog polymorphism and simple sequence repeat markers were used to identify markers linked to the resistance QTL. Two QTL, QYr.nafu2BL and QYr.nafu-3BS conferring APR to stripe rust, were mapped to 8.8 and 3.3 cM intervals on chromosome 2BL and 3BL, respectively. QYr.nafu-2BL is likely a new APR gene for resistance to stripe rust, and

QYr.nafu-3BS is likely the same locus as previously identified QYr.uga-3BS.1. These QTL with linked markers could be used in developing wheat cultivars with stripe rust resistance.

Keywords Durable resistance  Puccinia striiformis f. sp. tritici  Quantitative trait locus  Triticum aestivum  Yellow rust

Introduction

Global wheat (Triticum aestivum L.) production is hampered by several biotic stresses, including three rust diseases (http://www.globalrust.org). Stripe rust (yellow rust), caused by Puccinia striiformisWestend. f. sp. tritici Erikss. (Pst), is one of the most widely distributed wheat diseases in the world. Stripe rust causes significant economic losses in terms of reduced production and/or high costs for the chemical control of the disease (Wellings 2007; http://www.globalrust. org). Although fungicides can control stripe rust, growing resistant cultivars is a more economic and effective approach to control this disease (Line 2002;

Chen 2005, 2013, 2014).

X. L. Zhou and D. J. Han have contributed equally to this work.

X. L. Zhou  D. J. Han  J. M. Mu  W. B. Xue 

Q. D. Zeng  Q. L. Wang  L. L. Huang  Z. S. Kang

State Key Laboratory of Crop Stress Biology in Arid

Areas, Northwest A&F University,

Yangling 712100, Shaanxi, China

D. J. Han  J. M. Mu  W. B. Xue

College of Agronomy, Northwest A&F University,

Yangling 712100, Shaanxi, China

X. M. Chen

US Department of Agriculture, Agricultural Research

Service, Wheat Genetics, Quality, Physiology and Disease

Research Unit, and Department of Plant Pathology,

Washington State University, Pullman, WA 99164-6430,

USA

Q. D. Zeng  Q. L. Wang  L. L. Huang  Z. S. Kang (&)

College of Plant Protection, Northwest A&F University,

Yangling 712100, Shaanxi, China e-mail: kangzs@nwsuaf.edu.cn 123

Euphytica

DOI 10.1007/s10681-015-1447-7

Seedling (or all-stage) resistance conferred by racespecific genes is widely deployed because this type of resistance is often used in wheat breeding programs and rapidly selects predominant races that overcome the resistance genes (McDonald and Linde 2002; Ren et al. 2012a). The effectiveness of race-specific genes is usually short-lived in the natural presence of pathogen races with matching virulence alleles (Singh et al. 2004; Wan et al. 2004; Chen 2007, 2013; Singh et al. 2011; Rosewarne et al. 2012). The phenomenon of the loss of effectiveness of such genes or their combinations led scientists to search for alternative approaches to control the disease. Adult-plant resistance (APR), including high-temperature adult-plant (HTAP) resistance, is controlled by single or multiple genes and is gaining popularity in breeding programs due to its relative durability compared with all-stage resistance (Line 2002; Lu et al. 2009; Ren et al. 2012a, b, c; Basnet et al. 2013). APR resistance has been widely used in breeding wheat cultivars with resistance to stripe rust in the U.S. and many countries in the world, and many cultivars has exhibited resistance for 20–40 years (Qayoum and Line 1985; Chen 2005, 2013).

Currently, sixty-seven genes for stripe rust resistance have been cataloged (Basnet et al. 2013; Xu et al. 2013; McIntosh et al. 2013, 2014; Zhou et al. 2014).

Several permanently named resistance genes, Yr11,

Yr12, Yr13, Yr14, Yr16, Yr18, Yr29, Yr30, Yr34, Yr36,

Yr39, Yr46, Yr48, Yr52, Yr54, Yr59 and Yr62, confer adult plant resistance, whereas the others confer allstage resistance. Marker-assisted selection (MAS) allows breeders to select or to identify the presence of multiple genes. With the development of modern molecular mapping techniques during the last 15 years, many APR quantitative trait loci (QTL) for stripe rust resistance have been identified with the help of molecular markers. These genes have been mapped on all 21 chromosomes (He et al. 2011; Basnet et al. 2013; Chen 2013; Rosewarne et al. 2013). Recently,

Chen (2013) and Rosewarne et al. (2013) summarized more than 140 QTL for resistance to stripe rust in wheat. Many of the QTL have been used in breeding programs, and molecular markers closely linked to these QTL have greatly facilitated selection for APR in breeding programs.

P9897 (CI 14312, P-9897-8T-2B-1T-1B) is a spring wheat line developed by CIMMIT (http://www. wheatpedigree.net/sort/show/53708). In our germplasm evaluation, P9897has exhibited a high level of resistance to stripe rust in the field, although it is susceptible to

Chinese Pst races CYR29, CYR32, CYR33 and PSTCH42 at the seedling stage, indicating typical APR to stripe rust (Kang et al. unpublished data). The objectives of this study were to map QTL for stripe rust resistance with molecular markers, determine the stability of detected QTL across different environments and assess the combined effects of these QTL on the reduction of stripe rust severity.

Materials and methods

Plant materials

Seed of P9897 was provided by Dr. Wanquan Ji at

Northwest A&FUniversity. Mingxian 169 (M169) is a