Dissecting root proteome of transgenic rice cultivars unravels metabolic alterations and accumulation of novel stress responsive proteins under drought stressby Soumitra Paul, Dipak Gayen, Swapan K. Datta, Karabi Datta

Plant Science


Agronomy and Crop Science / Plant Science / Genetics


Please ci metabolic http://dx.


Plant Science xxx (2015) xxx–xxx

Contents lists available at ScienceDirect

Plant Science j ourna l ho me pa ge: www.elsev ier .com/ locate /p lantsc i

Dissecting root proteome of transgenic rice cultiv metabolic alterations and accumulation of novel proteins under drought stress

Soumitra tta ∗Q1

Laboratory of T ta, 35 Q2

West Bengal, In a r t i c l

Article history:

Received 17 D

Received in re

Accepted 11 February 2015

Available online xxx


DREB1A overexpressing transgenic rice




Two-dimensio lants owev tood. proteome analysis between the roots of wild type and transgenic DREB1A overexpressing homozygous plants under drought stress condition. After 7 d of dehydration stress at reproductive stage, the plants were re-watered for 24 h. The roots were collected separately from wild type and transgenic plants grown under water, drought stress and re-watering conditions and total proteins were analyzed by two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS). Among the large number of differentially accumulated spots, 30, 27 and 20 spots were successfully identified as differentially expressed proteins in three different conditions respectively. The major class of identified proteins 1. Introdu


Food sec alleviate th 40% of the demand in f abiotic stre population, land. Rain-f

Southeast A

Abbreviatio dehydration-r dimensional g

GDP, Guanosi ionization-tim ∗ Correspon

E-mail add 1 Equal cont http://dx.doi.o 0168-9452/© 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31te this article in press as: S. Paul, et al., Dissecting root proteome of transgenic rice cultivars unravels alterations and accumulation of novel stress responsive proteins under drought stress, Plant Sci. (2015), doi.org/10.1016/j.plantsci.2015.02.006 nal gel electrophoresis belongs to carbohydrate and energy metabolism category while stress and defense related proteins are especially up-accumulated under drought stress in both the plants. A novel protein, R40C1 was reported to be up-accumulated in roots of transgenic plants which may play an important role in generation of drought tolerant plants. Protein–protein interaction helps to identify the network of drought stress signaling pathways. © 2015 Published by Elsevier Ireland Ltd. ction urity is a major challenge in developing countries. To is problem, crop yield must to be increased. Globally, areas need to be cultivated by crops to meet the food uture where drought might be a major constrain among sses [1]. Rice, a staple food crop for half of the world’s is cultivated mainly in the irrigated and rain-fed low ed low land is the major harvesting area in South and sia which accounts for 90% of the globally cultivated ns: ABA, abscisic acid; SnRK2, SNF-related protein kinase 2; DREB, esponsive element-binding; ROS, reactive oxygen species; 2DE, two el electrophoresis; WT, wild type; IPG, immobilized pH gradient; ne diphosphate; MALDI-TOF MS, matrix assisted laser desorption e of flight mass spectrometry. ding author. Tel.: +91 339830538205; fax: +91 3324614849. ress: krbdatta@yahoo.com (K. Datta). ribution. rain-fed land. In Asia, about 50% of cultivated land is rain-fed although majority of grain yield comes from the irrigated land [2].

The cultivation in rain-fed land depends on rainfall. Inadequate rainfall causes drought. Recent climatic changes such as uncertainty of rainfall, improper intensity and distribution of water worsen the drought condition and severely affect rice production in rainfed lowland. In India, about 7.3 million ha of low land rice out of 20.4 million ha rain-fed rice areas are estimated to be droughtprone [3]. Severe affect of water stress in rice growth is commonly reflected from reduction of leaf area in vegetative stage [4]. The significant reduction during flowering stage is exerted by thwarted panicle exertion, perturbation of anthesis and spikelet desiccation, abnormal pollination and loss of viable pollen grain has been reported due to water scarcity during flowering [5]. Improvement of drought resistance has major impact on rice biomass production.

The complex signaling responses by plants during drought stress are indicated by morphological, biochemical and physiological alteration including higher transpiration rate and wilting, reduced rg/10.1016/j.plantsci.2015.02.006 2015 Published by Elsevier Ireland Ltd. 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 Paul1, Dipak Gayen1, Swapan K. Datta, Karabi Da ranslational Research on Transgenic Crops, Department of Botany, University of Calcut dia e i n f o ecember 2014 vised form 5 February 2015 a b s t r a c t

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