Anticancer and immunostimulating activities of a novel homogalacturonan from Hippophae rhamnoides L. berryby Hailiang Wang, Tingting Gao, Yuzhi Du, Hongxia Yang, Lixin Wei, Hongtao Bi, Weihua Ni

Carbohydrate Polymers

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Year
2015
DOI
10.1016/j.carbpol.2015.06.021
Subject
Organic Chemistry / Materials Chemistry / Polymers and Plastics

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Text

Carbohydrate Polymers 131 (2015) 288–296

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Anticancer and immunostimulating activities of a homogalacturonan from Hippophae rhamnoides L

Hailiang Wanga,1, Tingting Gaob,c,1, Yuzhi Dub,c, Hongxia Yan

Hongtao b,d,∗ e,∗∗ a Department o b Qinghai Key L te of P

Xining 810008 c Key Laborator y of S d Key Laborato Chine e Department o ina a r t i c l e i n f o

Article history:

Received 3 April 2015

Received in re

Accepted 6 Jun

Available onlin

Keywords:

Homogalactur

Hippophae rha

Antitumor act

Immunostimu

Chemical comp

Galacturonic a d-Glucose (Pu d-Galactose (P l-Arabinose (P d-Mannose (P l-Rhamnose (P l-Xylose (PubC a b s t r a c t

Our previous study isolated an anti-fatigue polysaccharide (HRWP) from the Hippophae rhamnoides berry.

In this study, using ion-exchange chromatography and gel filtration chromatography in turn, a water1. Introdu

Current because of rides have b and their en possible me ∗ Correspon macology and

Academy of Sc fax: +86 971 6 ∗∗ Correspon

Science, Jilin U fax: +86 431 8

E-mail add 1 These auth http://dx.doi.o 0144-8617/© vised form 2 June 2015 e 2015 e 14 June 2015 onan mnoides L. ivity lating activity ounds studied in this article: cid (PubChem CID: 441476) bChem CID: 5793) ubChem CID: 6036) ubChem CID: 5460291) ubChem CID: 18950) ubChem CID: 19233) hem CID: 95259) soluble homogenous polysaccharide HRWP-A was isolated from HRWP. Structural analysis determined that HRWP-A was a polysaccharide with repeating units of (1 → 4)--d-galactopyranosyluronic residues, of which 85.16% were esterified with methyl groups. An antitumor activity assay showed that HRWP-A could significantly inhibit the Lewis lung carcinoma (LLC) growth in tumor-bearing mice. Further experiments suggested that the antitumor effect of HRWP-A might be mediated through immunostimulating activity, as it enhances the lymphocyte proliferation, augments the macrophage activities, as well as promoting NK cell activity and CTL cytotoxicity in tumor-bearing mice. To our knowledge, this is the first report on a natural antitumor high-methoxyl homogalacturonan pectin from the H. rhamnoides berry—a compound that acts as a potential immunostimulant and anticancer adjuvant. © 2015 Elsevier Ltd. All rights reserved. ction therapies against cancer are still unsatisfactory, mainly their side effects. In recent years, natural polysacchaeen described as biological response modifiers (BRMs), hancement of the host defense has been recognized as a ans of inhibiting tumor growth with no harm to the host ding author at: Qinghai Key Laboratory of Tibetan Medicine PharSafety Evaluation, Northwest Institute of Plateau Biology, Chinese iences, Xining 810008, China. Tel.: +86 971 6143765; 143765. ding author at: Department of Immunology, College of Basic Medical niversity, Changchun 130021, China. Tel.: +86 431 85619476; 5619476. resses: bihongtao@hotmail.com (H. Bi), 65559211@qq.com (W. Ni). ors contributed equally to this work. (Leung, Liu, Koon, & Fung, 2006). The activity of polysaccharides might be caused and influenced by their glycosidic linkages, chain length, the number of branched points, molecular size and tertiary structure (Raveendran Nair et al., 2004). The unique structure diversities and physiochemical properties can be utilized successfully in various medical applications, and many polysaccharides have shown promising potential as antitumor agents, such as -glucans, amylopectin-like polysaccharides and pectins (Brown, 2006; Cao, 2013; Jin, Zhao, Huang, & Shang, 2014; Silva et al., 2012; Sun, 2011). These polysaccharides usually have low toxicity and few side effects, which make them appropriate for immunotherapy against tumors.

The Hippophae rhamnoides L. berry has been a traditional medicinal food of the Tibetan plateau for thousands of years that is recorded in the classic Tibetan pharmacological book “Crystal

Pearl of Materia Medica” (Chinese pinyin: jingzhubencao) and has been used for relieving cough, aiding digestion, invigorating rg/10.1016/j.carbpol.2015.06.021 2015 Elsevier Ltd. All rights reserved. Bi , Weihua Ni f Neurosurgery, The Second Hospital of Jilin University, Changchun 130033, China aboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institu , China y of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academ ry of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, f Immunology, College of Basic Medical Science, Jilin University, Changchun 130021, Ch novel . berry gb,c, Lixin Weib,c, lateau Biology, Chinese Academy of Sciences, ciences, Xining 810008, China se Academy of Sciences, Xining 810000, China

H. Wang et al. / Carbohydrate Polymers 131 (2015) 288–296 289 blood circulation and alleviating pain since ancient times (Dierma, 2012; Ma et al., 2011). To date, studies on this berry have mainly focused on small molecular compounds of the H. rhamnoides berry, but very few studies have reported on its polysaccharide content (Dongowsk (2001) isola branches fr research gr from H. rha of homogal (Ni et al., 2 features an charide from analysis an performed so. This and tinctive reso 2. Materia 2.1. Materi

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The isol berries wa (Ni et al., 2 exhaustivel compounds lected, drie filter paper to a small added to th rides, which

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HRWP w matography two fractio further pur and DEAE-S turn to gen and pharma

The tot phenol–H2S the m-hydr

Asboe Han content ass filtration ch drate and u by a method published by Sedmak and Grossberg (1977), with